Library Data




Here is information about the human ORFeome library data. You can find information about the vectors used for these libraries here.

Human Library Data


Table I. Summary of Vectors and Flanking Sequences in ORFeome Clones (10/1/08)
(Sequences read from 5' (from the promoter) to 3' as they would appear in the vector)
Clone
Source 		Donor
Vector		 Entry
Vector
(type att site)		 5' End of attL1.x + Seq to ATG 3' End of ORF to attL2.x
MGC
Synthetic 		pDONR223.1 pENTR223.1
(att1-att2)
SpnR 		-G TAC AAA AAA GCA GAA GGG CCG TCA AGG CCC ACC ATG
Y K K A E G P S R P T 		-NNN-TAA/G GGC CTC ATG GGC CCA GCT TTC TTG TAC
* G L M G P A F L Y
MGC
Synthetic 		pDONR223.1 pENTR223.1
(att1-att2)
SpnR 		-G TAC AAA AAA GCA GAA GGG CCG TCA AGG CCC ACC ATG
Y K K A E G P S R P T 		-NNN-TCA GGC CTC ATG GGC CCA GCT TTC TTG TAC
S G L M G P A F L Y
WTSI pDONR223 pENTR223
(att1.1-att2.1)
SpnR		 -G TAC AAA AAA GTT GGC ACC ATG
Y K K V G T 		-NNN-CCA ACT TTC TTG TAC-
P T F L Y
DFCI-
CCSB
(ORFeome 1&3) 		pDONR223 pENTR223
(att1.1-att2.1)
SpnR		 -G TAC AAA AAA GTT GGC ATG
Y K K V G 		-TA/GC CCA ACT TTC TTG TAC- TRC replaces stop
P T F L Y
DFCI-
CCSB
(ORFeome 5&7) 		pDONR223 pENTR223
(att1.1-att2.1)
SpnR		 -G TAC AAA AAA GTT GGC ACC ATG
Y K K V G T 		-NNN-TTG CCA ACT TTC TTG TAC- TTG replaces stop
L P T F L Y
HIP pDONR221 pENTR221
(att1-att2)
KnR		 -G TAC AAA AAA GCA GGC TCC ACC ATG
Y K K A G S T 		-TAG-GAC CCA GCT TTC TTG TAC
* D P A F L Y
HIP pDONR221 pENTR221
(att1-att2)
KnR		 -G TAC AAA AAA GCA GGC TCC ACC ATG
Y K K A G S T 		-TTG-GAC CCA GCT TTC TTG TAC
L D P A F L Y
Kazusa pDONR201 pENTR201
(att1-att2)
KnR		 -G TAC AAA AAA GCA GGC TTC GAA GGA GAT AGA ACC ATG
Y K K A G F E G D R T 		-TAA GTA GAC CCA GCT TTC TTG TAC
* V D P A F L Y
Kazusa pDONR201 pENTR201
(att1-att2)
KnR		 -G TAC AAA AAA GCA GGC TTC GAA GGA GAT AGA ACC ATG
Y K K A G F E G D R T 		-TAC GTA GAC CCA GCT TTC TTG TAC
Y V D P A F L Y
DKFZ pDONR221 pENTR221
(KnR)		 -G TAC AAA AAA GCA GGC TCC ACC ATG-
Y K K A G S T 		-TGA ATC CAC CCA GCT TTC TTG TAC-
* I H P A F L Y
DKFZ pDONR221 pENTR221
(KnR)		 -G TAC AAA AAA GCA GGC TCC ACC ATG-
Y K K A G S T 		-TGG ATC CAC CCA GCT TTC TTG TAC-
W I H P A F L Y
DKFZ pDONR221 pENTR221
(KnR)		 -G TAC AAA AAA GCA GGC TCC ACC ATG-
Y K K A G S T 		-AAG CTT GAC CCA GCT TTC TTG TAC-
K L D P A F L Y
DKFZ pDONR221 pENTR221
(KnR)		 -G TAC AAA AAA GCA GGC TCC ACC ATG-
Y K K A G S T 		-TGT ATT CAC CCA GCT TTC TTG TAC-
C I H P A F L Y
DKFZ pDONR221 pENTR221
(KnR)		 -G TAC AAA AAA GCT GGC ACC ATG-
Y K K A G T 		-GGC GAC CCA GCT TTC TTG TAC-
G D P A F L Y
DKFZ pDONR221 pENTR221
(KnR)		 -G TAC AAA AAA GCA GGC TCC ACC ATG-
Y K K A G S T 		-TAG CTT GAC CCA GCT TTC TTG TAC-
* L D P A F L Y
DKFZ pDONR221 pENTR221
(KnR)		 -G TAC AAA AAA GCA GGC TCC ACC ATG-
Y K K A G S T 		-TGA ATT CAC CCA GCT TTC TTG TAC-
* I P P A F L Y
DKFZ pDONR201 pENTR201
(KnR)		 -G TAC AAA AAA GCT GGC ACC ATG-
Y K K A G T 		-GGC GAC CCA GCT TTC TTG TAC-
G D P A F L Y
DKFZ pDONR201 pENTR201
(KnR)		 -G TAC AAA AAA GCT GGC ACC ACC ATG-
Y K K A G T 		-TAG GGC GAC CCA GCT TTC TTG TAC-
* G D P A F L Y
DKFZ None pENTR/D-
TOPO*
(KnR)		 -G TAC AAA AAA GCA GGC TCC GCG GCC GCC CCC TTC ACC ATG-
Y K K A G S A A A P F T 		-AGG GGT GGG CGC GCC GAC CCA GCT TTC TTG TAC-
K G G R A D P A F L Y

These vectors can be ordered from Invitrogen Corp.

Key to abbreviations: MGC: NIH Mammalian Gene Collection; WTSI: Wellcome Trust Sanger Institute;
HIP: Harvard Institute of Proteomics; DFCI-CCSB: Dana-Farber Cancer Institute, Center for Cancer Systems Biology;
Kazusa: Kazusa DNA Research Institute; DKFZ: German Cancer Research Center

* Stop codon (suppression potentially can yield C-terminal fusion proteins)