primate (rhesus monkey) cDNA Library Information

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Information for 4 libraries (by tissue)

brain/CNS

   1. NCI_CGAP_Brn72

ovary

   2. NICHD_Rh_Ov1

testis

   3. NICHD_Rh_Test1
   4. NICHD_Rh_Test2

NCI_CGAP_Brn72

Name: NCI_CGAP_Brn72
Library ID: 1807
Organism: rhesus monkey
Age: 0
Organ: brain/CNS
Tissue: hypothalamus
Host: DH10B
Vector: pCMV-SPORT6.ccdb
Vector type: phagemid
Insert digest: 5' EcoRV/NotI 3'
Stop Codon Status: without
Description: Cloned unidirectionally. Primer: Oligo dT. Average insert size 2.2 kb. Constructed by Invitrogen (Carlsbad, CA).

NICHD_Rh_Ov1

Name: NICHD_Rh_Ov1
Library ID: 1999
Organism: rhesus monkey
Age: 0
Stage: adult
Organ: ovary
Host: DH10B TonA
Vector: pDNR-LIB
Vector type: plasmid
Insert digest: SfiI (directional)
Stop Codon Status: without
Description: Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.0-4.0 kb. Tissue pooled from pre-pubertal, post pubertal and menopausal monkeys. Constructed by Clontech Laboratories (Palo Alto, CA).

NICHD_Rh_Test1

Name: NICHD_Rh_Test1
Library ID: 2016
Organism: rhesus monkey
Gender: male
Age: 0
Stage: adult
Organ: testis
Host: DH10B TonA
Vector: pDNR-LIB
Vector type: plasmid
Insert digest: SfiI (directional)
Stop Codon Status: without
Description: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT (30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.67 kb (range 0.8-3.5 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).

NICHD_Rh_Test2

Name: NICHD_Rh_Test2
Library ID: 2017
Organism: rhesus monkey
Gender: male
Age: 0
Stage: juvenile
Organ: testis
Host: DH10B TonA
Vector: pDNR-LIB
Vector type: plasmid
Insert digest: SfiI (directional)
Stop Codon Status: without
Description: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.67 kb (range 0.8-3.5 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).